Composite

Part:BBa_K4156127:Design

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-10)


HlpA with INP-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 194
    Illegal NgoMIV site found at 276
    Illegal NgoMIV site found at 527
    Illegal AgeI site found at 855
    Illegal AgeI site found at 1508
    Illegal AgeI site found at 1620
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To export the HlpA-binding protein to the EcN surface, a (truncated) INP (ice nuclease protein) tag is fused to the N-terminal end of HlpA.Also, we fused 6x his tag at the end of Hlpa to facilitate purification. And to visualize bacterial adhesion experiments,We added the red fluorescent protein after HlpA.


Source

Experimental Constructed

References